91 1027-9520 Iranian Blood Transfusion Organization Research Center 1086 Epidemiolgy & Statistical Effective factors on blood donation in the city of Kerman (2009-2013) Daneshi S. b Molavi Vardanjani H. Jahangiri Mehr F. d Rezabeigi Davarani E. b 8 d 8 1 5 2017 14 2 77 83 30 10 2016 11 03 2017 Abstract Background and Objectives Nowadays, the use of blood and blood products has increased; thus, the efforts should be made to raise the number of  blood donors. The aim of this study was to analyze the determinants of blood donation in the city of  Kerman. Materials and Methods This is a prospective study in which 2010 new first time voluntary blood donors having referred to Kerman Blood Center in the year 2009 participated; they were evaluated for the factors effective on the blood donation attempt from then until 2013. Then, the statistical analysis was performed using SPSS software version 21. Results Out of the total number of participants, 50.1%, 33.7% and 16.2% were first-time, experienced, and regular donors, respectively. The mean and the standard deviation of the duration of blood donation attempts in monthly intervals and the frequency of  blood donation attempts within the same period were 35.19 ± 20.72 and 5.36 ± 3.89, respectively. The correlation between the age and the level of education of blood donors and regular blood donation was statistically significant (p < 0.05), but there was no significance among other variables (p > 0.0.5). Conclusions  Given the significant effect of high level education status, high age, and the marital status on the regular blood donation, it is imperative for the groups with these attributes to be considered for being changed into regular blood donors; the necessary education and culture promoting efforts should be made together with adequate praising measures to assure the continuing of regular blood donors.
1106 Imunology The allele frequencies of human Neutrophil Antigens 5 (HNA-5) in Tabriz city khosravi Atieh Shams Asanja Karim Shaiegan Mojgan Samiei Shahram i Ataee Zahra Abdollahi Maryam Kavari Mahnaz shafiei almasian Mahboobeh Hosseinzadeh Parisa i High Institute for Research & Education in Transfusion Medicine 1 5 2017 14 2 84 91 29 01 2017 24 04 2017 Abstract Background and Objectives Human neutrophil antigens include 5 antigen systems. These antigens are on the plasma membrane of white blood cells. The aim of the study is to know about HNA allele frequency in the population in order to estimate the prevalence of the diseases related to HNA-5. Materials and Methods In this descriptive study, blood samples were taken from 190 Azari unrelated blood donors having referred to Tabriz Blood Center. DNA extraction was performed using commercial kit Pars Tous. HNA-5a and HNA-5b molecular were then evaluated by PCR-RFLP. PCR products were digested using enzymes Sdu1 Bsp1286I.  HNA-5a+ and HNA-5a- (or HNA-5b) antigen gene frequencies were calculated using the Hardy-Weinberg equation. Results HNA-5a (+/+), HNA-5a (+/-) and HNA-5a (-/-) phenotypes were observed in 93, 10 and 87 patients, respectively. The observed frequencies were consistent with Hardy-Weinberg equilibrium; allele frequencies of HNA-5a+ and HNA-5a- (or HNA-5b) were equal to 0.51 and 0.49, respectively. Conclusions HNA-5 alleles frequency rates were in the same range reported for the other populations. 1083 Hematology The detection of Scavenger Receptor-B1 expression and its role on the function of platelets in patients with atherosclerotic disease Abasalizadeh Mahnaz Nehzati Prisa Allahbakhshian Mehdi Hamidpour Mohsen Khadem Mabudi Ali Akbar Tabatabaie Mohamad Reza 1 5 2017 14 2 92 100 25 10 2016 01 02 2017 Abstract Background and Objectives SR-B1 acts as the main receptor for HDL and plays an important role in reverse cholesterol transport. In contrast to LDL level which causes Atherosclerotic plaque in artery, plasma concentration of HDL correlates inversely with the incidence of coronary artery disease and atherosclerosis. This study investigates the expression of Scavenger Receptor-B1 and its role on the function of platelets in patients with atherosclerotic disease. Materials and Methods The present case-control study evaluated the SR-B1 receptor of 24 patients with atherosclerotic plaque and 24 healthy volunteers as the normal control. After separation of platelet, the expression of SR-BI was detected using western blotting. Platelet function in response to agonists was examined by aggregometry. Finally, the data were statistically analyzed using SPSS version 16 software and the student T-test. Results The western blotting assay showed a significant reduction of SR-BI protein expression in AD patients (0.4 AU) compared with the healthy control (1.4 AU), (p < 0.001). The platelet aggregation in response to ADP showed a significant increase in patients (85%) compared to the control (51%), (p < 0.001). Conclusions  The levels of SR-B1expression correlate inversely with platelet aggregation. 1091 Hematology Evaluation of prevalence of JAK 2 exon 12 gene mutations among Iranian patients with Polycythemia Vera Torab Zahra Poopak Behzad Movassagh Pour Ali Akbar Younesi M.R. Emami A.H. Elahi Fazel 1 5 2017 14 2 101 108 07 11 2016 16 01 2017 Abstract Background and Objectives Polycythemia Vera (PV) is one of the negative BCR-ABL1 myeloproliferative neoplasms arising from hematopoietic progenitors. About 3-4% of PV patients have some mutations in the exon 12 of JAK2 gene. Considering the importance of these mutations in diagnosis of patients with PV, the present study was conducted. Materials and Methods In this descriptive analytical study, JAK2 exon 12 mutations were evaluated in 58 patients with diagnosis of PV in Payvand Laboratory and all of them were negative for V617F mutation. In addition, we had 60 detailed patient files who had V617F mutation. After quality control of extracted genomic DNA, the exon 12 were amplified by PCR. The screening for mutations was performed by direct DNA sequencing. The data were analyzed by SPSS 13 & c2 test. Results In this study 58 V617F-negative PV patients were studied. 45 (77.6%) cases were male and 13 (22.4%) were female. The mean age of patients was 46.8 ± 4.2. After needed analysis Mutation (E543-D544del) was seen in a 72 years old woman . Conclusions  There is low frequency of exon 12 mutation in Iranian population which can be as a result of low number of patients or low frequency in Iranian population. So study on larger population should be informative.  1104 Molecular Genetic Prevalence of various mutations in Beta thalassaemia in Province of Chahar Mahal Bakhtiari and Isfahan and its association with haematological parameters Heidari Soureshjani E. Vallian S. Mirahmadi Babaheidari S.F. Abasian F. 1 5 2017 14 2 109 117 15 01 2017 15 04 2017 Abstract Background and Objectives There is considerable phenotypic variation of Beta-thalassemia with common mutations whose understanding would facilitate the prevention of the specific syndromes. The aim of this study was to determine the common Beta-thalassemia mutations in Isfahan and Chaharmahal Bakhtiari province and thier relationship with blood parameters. Materials and Methods In this descriptive study, 10 ml venous blood samples were taken from 321 Beta thalassemia carriers having referred to the private Isfahan Center of Medical Genetics in 2016. For the confirmation of the diagnosis of Beta thalassemia in these patients, the indices of MCV and MCH with Mindary device and HbA1, HbA2, Hbf and RBC with the electrophoresis method were measured. c2, t-test and SPSS 22 were used in analyzing data. Results Mutation Fr36/37 (-T) in the population studied in Isfahan and Chaharmahal Bakhtiari with the frequency rates of 34 (26.35%) and 22 (32.35%) showed the highest in the studied mutations. About 80% of cases were detectable mutations in the beta globin gene in the people with HbA2 > 3.5 and in 100% of cases with MCH < 27 and MCV < 80. Conclusions  Beta-thalassemia mutations among Chaharmahal Bakhtiari and Isfahan populations show diversity and wide distribution. Average mutations studied based on blood indices showed a wide variety. The results showed a positive correlation between beta-thalassemia mutations and red blood cells indices which can be effective in fast and efficient screening of these common mutations. 1055 Hematology and Oncology Frequency of Chromosome Disorders in the Childrenwith Acute Leukemia Referred to the OncSanandaj Besat Hospitalology Ward of Moradveisi B. Safari H. Roshani D. Khalafi B. Raghizadeh Sarvestani R. 1 5 2017 14 2 118 125 03 07 2016 17 01 2017 Abstract Background and Objectives Blood Malignancies are the most frequent cancers in children including about 45% of diagnosed cancers in children. Chromosome disorders are found in most of patients with acute lymphoblastic leukemia. These disorders present valuable information for prognostic determinants. Materials and Methods This descriptive study includes all the children with acute leukemia having referred to the oncology ward of Besat Hospital of Sanandaj from 1385 to the end of the first half of 1394. Peripheral blood smear and bone marrow aspirations were taken from the children; RT-PCR method was used for determination of chromosome karyotypes so as to detect chromosome disorders. The data were analyzed by SPSS 11.5 & c2 test. Results Among 75 cases, 12 chromosome genotypes were observed. Normal chromosome genotype has the highest frequency with 41 cases (54.7%). t(12-21) chromosome disorder observed in 15 cases (20%) was the most frequent disorder. t(15-17) chromosome disorder observed in 5 cases (6.5%) was the next most frequent disorder. The only chromosome disorder observed in T lymphocyte leukemia was 47xy + 21 with the frequency of 1 case. Conclusions Based on the research findings, 54.7% of the children have no chromosome disorders. Different results are reported in different researches. Due to the importance of finding the types of chromosome translocation for prognostic determinants in children with leukemia, more research is required. 1082 Hematology Cytotoxic effect of PI3Kδ inhibitor in acute promyelocytic leukemia cells Dadashi M. Bashash D. 1 5 2017 14 2 126 134 25 10 2016 10 04 2017 Abstract Background and Objectives PI3K/Akt pathway plays a key role in cell growth and proliferation. Constitutive activation of this pathway is detectable in 50-70% of patients with acute promyelocytic leukemia (APL). Previous studies showed that PI3K activity in APL cells is mainly due to overexpression of p110δ  isoform. In an effort to investigate the effect of PI3K inhibition in acute promyelocytic leukemia, APL-derived NB4 cells were subjected to different concentrations of Idelalisib, as a potent p110δ-specific inhibitor. Materials and Methods In this experimental study, we examined the effect of Idelalisib on the viability and metabolic activity of NB4 cells. Moreover, flowcytometery and quantitative RQ-PCR were applied to investigate both induction of apoptosis and transcriptional activity of apoptosis-related genes, respectively. Results The results revealed that Idelalisib not only decreased cell viability and metabolic activity in a dose- and time-dependent manner, but also induced cell death through the apoptotic pathway in NB4 cells. Our data also delineated that Idelalisib-induced apoptosis was mediated through induction of Bax and PUMA coupled with decreased expression of Mcl-1. Conclusions Based on P110δ activity in APL patients and the potent efficacy of Idelalisib in NB4 cells, it is tempting to suggest this inhibitor, as either single agent or in combination with common medications, for the treatment of patients with APL. 1056 Hematology Correlation of congenital abnormalities with blood groups in children admitted to the pediatric surgery ward of Isfahan Alzahra Hospital in 2008-2014 Gholipour Shoaleh Gholipour shirin Zargham Ali Isfahan University 1 5 2017 14 2 135 139 03 07 2016 27 02 2017 Abstract Background and Objectives The correlation between ABO blood group distribution and congenital abnormalities has not been evaluated in the past studies. This study investigated the correlation between the frequency of congenital abnormalities and ABO/Rh blood groups in a sample population over a period of 6 years. Materials and Methods In this descriptive and retrospective study, the ABO and Rh blood groups of 455 admitted children were determined in Alzahra Hospital, Isfahan, Iran, from 2008 to 2014. Demographic data were collected in the medical record unit. We analyzed the demographic data and ABO/Rh frequencies based on the patients file information by SPSS 16, c2 and t-Test. Results In this study, we observed an overall trend of anomaly in both male and female newborns: with the urinary tract anomalies in 341 male newborns (74.9%) and the head and neck defects in 114 female newborns (25.1%). The anomalies in the gastrointestinal and urinary systems of Rh-negative children and the facial anomalies in Rh-positive children were more significantly frequent compared to others. Conclusions   There was a significant positive correlation between the Rh distribution and congenital abnormalities; however, no significant correlation was observed between ABO blood groups and anomalies in different organs. 1073 Hematology Comparison of the effects of omega-3 supplements and L-carnitine with the combination exercise on hematological parameters of type 2 diabetic women Hassani Ali Ghorbani Masoumeh Shahrood University of Technology 1 5 2017 14 2 140 146 30 09 2016 27 02 2017 Abstract Background and Objectives The purpose of this quasi-experimental study was to compare the effects of 8 weeks of supplementation with omega-3 and L-carnitine with the combination exercise on hematology parameters of type 2 diabetic women. Materials and Methods In this semi-experimental study, 58 patients with type 2 diabetes having referred to Diabetes Association of Shahrood with the mean age of 52.7 ± 6.17 years were randomy divided into six groups: (1) exercise + placebo [10 patients], (2) exercise + omega-3 [10 patients], (3) exercise + L carnitine [10 patients], (4) exercise+omega 3 + L carnitine [10 patients], (5) omega-3 + Lcarnitine [10 patients], and (6) the control group [8 patients]. The patients underwent endurance-resistance exercises. Results The number of WBCs in groups 1, 3, and 4  and that of HCT in the groups 3 and 4 compared to the control showed a significant decrease. However for RBCs, a significant increase in the groups 2, 3, and 4  compared to the control group was observed. Conclusions  It seems that 8 weeks of supplementation with omega-3 and L-carnitine combined with the combination exercises place an impact on the amount of WBCs and RBCs improving the oxygen carrying capacity in the patients with type 2 diabetes. 1128 Blood donation The Recruitment and Retntion of Blood Donors Maghsudlu M. Pourfathollah A.A Khosravi s. Asadi S. Mahdaviani F.S. Tabatabaie M. Mehdizadeh M. Hosseini M. Mirzaie R. Zareie I Zeinali M. Soltanian E. Adeli O.A. Ghasemzadeh A. Rezazadeh B. Ghahraman Rezaieyeh M. Moradi A. Noorian Bidgoli M. Baradarn M.H. Mamvari K. Karimi M.H. Mehran M. 1 5 2017 14 2 147 154 19 06 2017 19 06 2017 ندارد ندارد ندارد ندارد ندارد ندارد ندارد ​ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ​ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ​ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ​ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ​ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ​ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ​ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ​ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ​ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ندارد ​ندارد ندارد ندارد