Platelet compatibility assessment between AML patients and platelet donors by flow cytometry
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M. Sayyadi , M. Shaiegan , M. Nikouguftar , M. Vaezi , A. Malek-Mohammadi , M.H. Ahmadi , S. Mohammadi , M. Zadsar |
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Abstract: (6326 Views) |
Abstract Background and Objectives One of the complications of repeated platelet transfusion is immune platelet refractoriness. In this study, a flow cytometic platelet cross-matching was performed to evaluate platelet transfusion outcome. Materials and Methods In this descriptive study, fifteen patients with a history of multiple platelet transfusions and fifteen healthy participants as the control group were enrolled in this study. Platelet cross-match was done and analyzed by the gate method. EDTA-anticoagulated blood samples from healthy donors were collected and PRP sampls were obtained. The serum of each patient was added to platelet suspension and incubated with FITC-anti human IgG and was analyzed using a flow cytometer. Platelet cross-match results of the patients were compared with those of CCI. Results Platelet cross match results were negatively correlated with 1-hour (r = -0.731, p = 0.002) and 24-hour (r = -0.794, p = 0.001) CCIs. There was a significant difference in the mean percentage of platelet crossmatch between the patients with immune platelet refractoriness (showing 1-hour CCIs less than 7500) and that of the control group and the patients without platelet refractoriness. Conclusions The results showed that there was a significant correlation between platelet cross match by flowcytometry and 1-hour CCI results and this method could be applicable in the evaluation of platelet transfusion outcome. |
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Keywords: Key words: Flow Cytometry, Crossmatching٬ Blood, Platelet-Rich Plasma |
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Type of Study: Research |
Subject:
Imunohematology Published: 2016/03/14
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