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Detection of the Lepore and Asian-Indian beta-globin gene deletions with duplex-PCR
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F. Rahmi Nezhad   , R. Ali Mohammadi , P. Fuladi , S. Foroughi , M. Feiz Pour , R. Vahidi , M. Hashemi , F. Mola Zadeh , M. Heidari , M. Deilam Salehi , S. Zeinali |
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Abstract: (15614 Views) |
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Background and Objectives
Beta-thalassemia is one of the most common single-mutation diseases. These mutations cause decreased beta-globin protein synthesis or even deletion. Most of the mutations in beta-globin gene are point mutations but there are some small and big deletions in beta-globin gene cluster. The goal of this research is to detect and study two deletions (Lepore, Asian-Indian) with duplex-PCR.
Materials and Methods
This study was done on 3 Asian-Indian deletion samples and 10 Lepore samples. After detecting two deletions (Lepore and Asian-Indian), we started setting Gap-PCR in these two known deletions. Duplex-PCR with 8 different primers for detecting Lepore and Asian-Indian deletions simultaneously was set at 68ºC annealing temperature these two deletions can be detected easily in one reaction with this new method.
Results
The results show that detecting Lepore and Asian-Indian deletions with multiplex PCR is practical, and the specific bands for each deletion are detectable.
Conclusions
Detecting these two deletions in one reaction (Duplex-PCR) is practical. This method is easy, reliable, and fast it is also economical and saves both time and money.
Key words: Beta-thalassemia, Beta-globins, Gene deletion |
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| Keywords: Beta-thalassemia, Beta-globins, Gene deletion |
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Full-Text [PDF 241 kb]
(3176 Downloads)
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Type of Study: Research |
Subject:
Genetis
Published: 2014/07/26
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