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Seroepidemiologic status of brucellosis in blood donors in Yazd, 2009
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R. Ghilian   , S.H. Hekmati Moghaddam , A. Fatemi , H. Eslamieh , M. Dargahi |
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Abstract: (23225 Views) |
| Abstract
Background and Objectives
The aim of this study was to determine the frequency of brucellosis and the titer of anti-brucella antibody by the Wright and ELISA methods in blood donors in Yazd province sensitivity and specificity rates of these two methods were also compared.
Materials and Methods
This descriptive/analytical cross-sectional study was performed on 300 healthy donors admitted to Yazd Blood Transfusion Center. All of them were interviewed, examined, and tested by routine serologic diagnostic tests of brucellosis, including rapid Wright, standard tube Wright test, 2-ME (2-mercapto ethanol), and ELISA (enzyme-linked immunosorbent assay).
Results
Nineteen (6.3%) out of 300 participants had a tube Wright titer of 1:80 (5.7% of men and 14.3% of women). By ELISA method, 17 persons (5.7%) had positive IgG anti-Brucella antibody. The 2-ME test was positive in only 2 cases (0.6%). The sensitivity, specificity, positive predictive, and negative predictive values of ELISA compared to Wright test were 5.3%, 94.7%, 6.3%, and 93.7%, respectively.
Conclusions
If a titer of 1:80 is defined to be the criterion of contamination with brucella, it should not be considered negligible in blood donors of Yazd.
Key words: Brucella, Blood Donors, ELISA, Iran |
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| Keywords: Brucella, Blood donors, ELISA, Iran |
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Full-Text [PDF 259 kb]
(3095 Downloads)
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Type of Study: Review Article |
Subject:
Blood donation
Published: 2014/07/26
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