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Molecular diagnosis of B cell Non-Hodgkin Lymphoma by evaluation of immunoglobulin heavy chain gene rearrangement
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Abolfazl Yosefian   , Behzad Poopak , Hassan Abolghasemi , Azita Azarkeivan , Mohammad Farhadilangerodi , Alireza Sadeghipour , Mojgan Jeyhonian , Mohammad Javadpourkhayat , Khandan Zare , Kobra Farahani , Mitra Salahmand |
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Abstract: (26793 Views) |
| Molecular diagnosis of B cell Non-Hodgkin Lymphoma by evaluation of immunoglobulin heavy chain gene rearrangement
Yousefian A.1(MS ), Poopak B.2(PhD), Abolghasemi H.1,3(MD), Azarkeivan A.1(MD),
Farhadi Langroudi M.1(MD), Sadeghipour A.4(MD), Jeyhonian M.4(MD),Pourkhayat M.J.4(MD),
Zare K.5(MD), Farahani K.2(BS), Salahmand M.6(BS)
1Iranian Blood Transfusion Organization-Research Center-Iran
2Islamic Azad University-Medical Branch. Tehran- Iran
3Baghiyatalah University of Medical Sciences-Molecular Biology Research Center, Tehran-Iran
4IranUniversity of Medical Sciences, Iran
5Shahid Beheshti University of Medical Sciences,Iran
6Payvand Medical Laboratory, Iran
Abstract
Background and Objectives
Rearrangement of V, D, and J segments of immunoglobulin heavy chain gene with inserted or deleted nucleotides within rearranged segments makes unique hypervariable regions (CDR-3). These regions can be used for evaluation of B cell clonality for the purpose of molecular diagnosis of Non-Hodgkin Lymphoma (NHL) and for confirmatory diagnosis in suspicious cases.
Materials and Methods
In this study, samples of 42 patients were collected from Taleghani, Baqhiyatalah, and Aliasghar hospitals out of this number, there were 22 patients with diagnosis of B cell NHL, 10 with reactive hyperplasia, and 10 with malignant lymphoma. After DNA extraction from formalin fixed paraffin embedded tissues, PCR was done using consensus primers for amplification of CDR-3 region. PCR products were analyzed after heteroduplex analysis using polyacrylamide gel electrophoresis and silver stain.
Results
Clonal patterns in group 1 (B cell NHL), 2 (reactive and follicular hyperplasia), and 3 (morphological diagnosis without immunohistochemistry) were observed in 77.2%, 0%, and 70% of patients, respectively.
Conclusions
Our findings are compatible with other international studies with minor differences. The diagnosis of B-cell lymphoid malignancy can frequently be substantiated by detecting clonal immunoglobulin heavy chain (IGH ) gene rearrangement.
Key words: Gene rearrangement, Non-Hodgkin Lymphoma, Immunoglobulin
SJIBTO 2008 5(3): 157-166
Received: 31 Aug 2007
Accepted: 8 Oct 2008
Correspondence: Poopak B., PhD of Hematology. Islamic Azad University- Tehran Medical Branch.
P.O.Box: 19295-1495 , Tehran, Iran, Tel: (+9821) 22006660 Fax : (+9821) 22264145
E-mail: bpoopak@yahoo.com |
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| Keywords: Gene rearrangement, Non-Hodgkin Lymphoma, Immunoglobulin |
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Full-Text [PDF 330 kb]
(10427 Downloads)
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Type of Study: Research |
Subject:
Hematology
Published: 2014/08/17
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