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:: Volume 15, Issue 2 (Summer 2018) ::
Sci J Iran Blood Transfus Organ 2018, 15(2): 120-132 Back to browse issues page
The effect of telomerase inhibitor MST-312 on apoptosis of acute lymphoblastic leukemia cell line NALM-6
N. Ghasemimehr , Z. Yazdani , A.R. Farsinejad Dr., R. Mirzaee Khalilabadi , A. Fatemi Dr. *
Abstract:   (306 Views)
Abstract
Background and Objectives
There have been continuous efforts in telomerase-targeted therapy for cancer treatment because telomerase expression is significantly increased in about 85- 90% of human cancers while its expression is usually silenced in normal cells.
 
Materials and Methods
Throughout this experimental study, NALM-6 cells were treated with different concentrations of telomerase inhibitor, MST-312 at different times. Then, cell viability and metabolic activity were evaluated by trypan blue and MTT assays; cell apoptosis was also evaluated by flow cytometry using Annexin V Apoptosis Detection Kit. In addition, the expression of BAX and BCL2 genes was evaluated in the treated cells using Quantitative Real-time PCR.
 
Results
A dose-and time-dependent decrease was observed in the viability of NALM-6 cells after exposure with different concentration of MST-312. Over 50% decrease was observed in the viability of the cells treated with 8 μM of MST-312 after 48 h. The cytotoxic effect of MST-312 was dose- dependent, with approximately 6, 19.69 and 56.9% reduction in metabolic activity of NALM-6 cells after 48 h exposure with 2, 4, and 8 μM of MST-312, respectively. Approximately 20% apoptosis was observed in NALM-6 cells treated with 4μM of MST-312 after 48 h.  Gene expression analysis also showed that 4μM of MST-312 led to upregulation of BAX and downregulation of Bcl-2 genes.
 
Conclusions 
Inhibition of telomerase activity by MST-312 can be proposed as a new candidate for the treatment of acute lymphoblastic leukemia.
 

 
Keywords: Key words: Telomerase, Apoptosis, Acute Lymphoid Leukemia
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Type of Study: Research | Subject: Hematology
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Ghasemimehr N, Yazdani Z, Farsinejad A, Mirzaee Khalilabadi R, Fatemi A. The effect of telomerase inhibitor MST-312 on apoptosis of acute lymphoblastic leukemia cell line NALM-6 . Sci J Iran Blood Transfus Organ. 2018; 15 (2) :120-132
URL: http://bloodjournal.ir/article-1-1195-en.html


Volume 15, Issue 2 (Summer 2018) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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