[Home ] [Archive]   [ فارسی ]  
:: Main :: About us :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Subscription::
News& Events::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Indexing
                        
..
:: Volume 3, Issue 4 (Winter 2007) ::
Sci J Iran Blood Transfus Organ 2007, 3(4): 291-298 Back to browse issues page
Carrier detection of hemophilia A in southern Khorasan using the 3 polymorphic sites of BclI, HindIII, and AlwNI
Mohammad Reza Abbaszadegan , Massod Ziaee , Farhad Khadivi-zand , Zahra Badiee , Bahram Khazaei , Zohreh Vahedian , Fahimeh Mehrabian , Ezat Dadkhah
Abstract:   (19649 Views)

  Abstract

 Background and Objectives

 Hemophilia A is the most common X-linked blood coagulation disorder. The prevalence rate of this disease in various communities is about 1-2/10000 males. The prevalence of hemophilia A is very high in southern Khorasan population, perhaps due to their isolation and high rate of consanguinity. The aim of this research was to detect hemophilia A carriers in two villages of southern Khorasan and set a prenatal diagnosis program for these families.

 

 Materials and Methods

 Blood samples of 34 patients with hemophilia A out of 51 family members (9 families) from two villages were collected. We were also able to collect samples from 7 mothers out of 9 families. Intragenic polymorphic sites in factor VIII gene including HindIII, Bc1I and AlwNI were used for carrier detection. DNA extraction and PCR-RELP were also performed.

 

 Results

 The results revealed 3 heterozygote mothers for HindIII, 2 for Bc1I and 1 for AlwNI. We utilized these polymorphic sites for carrier analysis in these families. Ultimately, 4 carrier sisters of affected boys in this population were found it was then possible to perform prenatal diagnosis procedure for their families.

 

 Conclusions

 HindIII and Bc1I polymorphisms can be suitable markers for hemophilia prenatal diagnosis in these two villages.

 

 Key words : Hemophilia A, RFLP (Restriction Fragment Length Polymorphism), Carrier detection

 

Keywords: Hemophilia A, RFLP (Restriction Fragment Length Polymorphism), Carrier detection
Full-Text [PDF 249 kb]   (3591 Downloads)    
Type of Study: Research | Subject: General
Published: 2014/08/18
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Abbaszadegan M R, Ziaee M, Khadivi-zand F, Badiee Z, Khazaei B, Vahedian Z, et al . Carrier detection of hemophilia A in southern Khorasan using the 3 polymorphic sites of BclI, HindIII, and AlwNI . Sci J Iran Blood Transfus Organ 2007; 3 (4) :291-298
URL: http://bloodjournal.ir/article-1-117-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 3, Issue 4 (Winter 2007) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
Persian site map - English site map - Created in 0.05 seconds with 39 queries by YEKTAWEB 4642