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:: Volume 15, Issue 1 (Spring 2018) ::
Sci J Iran Blood Transfus Organ 2018, 15(1): 28-35 Back to browse issues page
HOTAIR expression in newly diagnosed Acute Lymphoblastic Leukemia pateints
P. Fallah , M. Molaie , A. Ehsan Heidari , M. Soleimani , M. Jahedi Zargar , Ehsan Arefian , T. Madani , M. Asadi , B. Poopak
Keywords: Key words: Hematopoiesis, LncRNAs, Lymphocytic Leukemia, Acute
Full-Text [PDF 722 kb]   (1632 Downloads)     |   Abstract (HTML)  (4430 Views)
Type of Study: Research | Subject: Hematology
Published: 2018/03/11
Full-Text:   (1837 Views)
    References:
  1. Tomizawa D, Kiyokawa N. Acute Lymphoblastic Leukemia: Hematological Disorders in Children. USA: Springer; 2017. p. 33-60.
  2. Inaba H, Greaves M, Mullighan CG. Acute lymphoblastic leukaemia. Lancet 2013; 381(9881): 1943-55.
  3. Kahn JM, Keegan TH, Tao L, Abrahão R, Bleyer A, Viny AD. Racial disparities in the survival of American children, adolescents, and young adults with acute lymphoblastic leukemia, acute myelogenous leukemia, and Hodgkin lymphoma. Cancer 2016; 122(17): 2723-30.
  4. Fatica A, Bozzoni I. Long non-coding RNAs: new players in cell differentiation and development. Nat Rev Genet 2014; 15(1): 7-21.
  5. Esteller M. Non-coding RNAs in human disease. Nat Rev Genet 2011; 12(12): 861.
  6. Boon RA, Jaé N, Holdt L, Dimmeler S. Long Noncoding RNAs: From Clinical Genetics to Therapeutic Targets? J Am Coll Cardiol 2016; 67(10): 1214-26.
  7. Sigova AA, Mullen AC, Molinie B, Gupta S, Orlando DA, Guenther MG, et al. Divergent transcription of long noncoding RNA/mRNA gene pairs in embryonic stem cells. Proceedings of the National Academy of Sciences 2013; 110(8): 2876-81.
  8. Cesana M, Cacchiarelli D, Legnini I, Santini T, Sthandier O, Chinappi M, et al. A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA. Cell 2011; 147(2): 358-69.
  9. Mercer TR, Dinger ME, Mattick JS. Long non-coding RNAs: insights into functions. Nat Rev Genet 2009; 10(3): 155.
  10. Wu Y, Zhang L, Zhang L, Wang Y, Li H, Ren X, et al. Long non-coding RNA HOTAIR promotes tumor cell invasion and metastasis by recruiting EZH2 and repressing E-cadherin in oral squamous cell carcinoma. Int J Oncol 2015; 46(6): 2586-94.
  11. Huang L, Liao LM, Liu AW, Wu JB, Cheng XL, Lin JX, et al. Overexpression of long noncoding RNA HOTAIR predicts a poor prognosis in patients with cervical cancer. Arch Gynecol Obstet 2014; 290(4): 717-23.
  12. Svoboda M, Slyskova J, Schneiderova M, Makovicky P, Bielik L, Levy M, et al. HOTAIR long non-coding RNA is a negative prognostic factor not only in primary tumors, but also in the blood of colorectal cancer patients. Carcinogenesis 2014; 35(7): 1510-5.
 
  1. Hunger SP, Mullighan CG. Acute lymphoblastic leukemia in children. N Engl J Med 2015; 373(16): 1541-52.
  2. Rinn JL, Kertesz M, Wang JK, Squazzo SL, Xu X, Brugmann SA, et al. Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs. Cell 2007; 129(7): 1311-23.
  3. Swain A, Inoue T, Tan KS, Nakanishi Y, Sugiyama D. Intrinsic and extrinsic regulation of mammalian hematopoiesis in the fetal liver. Histol Histopathol 2014; 29(9): 1077-82.
  4. Liu XH, Sun M, Nie FQ, Ge YB, Zhang EB, Yin DD, et al. LncRNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer. Mol Cancer 2014; 13(1): 92.
  5. Kim K, Jutooru I, Chadalapaka G, Johnson G, Frank J, Burghardt R, et al. HOTAIR is a negative prognostic factor and exhibits pro-oncogenic activity in pancreatic cancer. Oncogene 2013; 32(13): 1616-25.
  6. Nakagawa T, Endo H, Yokoyama M, Abe J, Tamai K, Tanaka N, et al. Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer. Biochem Biophys Res Commun 2013; 436(2): 319-24.
  7. Qiu JJ, Lin YY, Ye LC, Ding JX, Feng WW, Jin HY, et al. Overexpression of long non-coding RNA HOTAIR predicts poor patient prognosis and promotes tumor metastasis in epithelial ovarian cancer. Gynecol Oncol 2014; 134(1): 121-8.
  8. Zhang YY, Huang SH, Zhou HR, Chen CJ, Tian LH, Shen JZ. Role of HOTAIR in the diagnosis and prognosis of acute leukemia. Oncol Rep 2016; 36(6): 3113-22.
  9. Hao S, Shao Z. HOTAIR is upregulated in acute myeloid leukemia and that indicates a poor prognosis. Int J Clin Exp Pathol 2015; 8(6): 7223-8.
  10. Nagano T, Fraser P. No-nonsense functions for long noncoding RNAs. Cell 2011; 145(2): 178-81.
  11. Kogo R, Shimamura T, Mimori K, Kawahara K, Imoto S, Sudo T, et al. Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers. Cancer Res 2011; 71(20): 6320-6.
  12. Loewen G, Jayawickramarajah J, Zhuo Y, Shan B. Functions of lncRNA HOTAIR in lung cancer. J Hematol Oncol 2014; 7: 90.
  13. Wu B, Ma H, Qu W, Wang B, Zhang X, Wang P, et al. Catalytic infrared and hot air dehydration of carrot slices. J Food Process Eng 2014; 37(2): 111-21.
  14. Yan TH, Lu SW, Huang YQ, Que GB, Chen JH, Chen
YP, et al. Upregulation of the long noncoding RNA HOTAIR predicts recurrence in stage Ta/T1 bladder cancer. Tumour Biol 2014; 35(10): 10249-57.
 
 
 


 
 
 
 
Sci J Iran Blood Transfus Organ 2018; 15(1): 28-35
Original Article
 

 

HOTAIR expression in newly diagnosed Acute Lymphoblastic
 Leukemia pateints
 
Fallah P.1, Molaie M.2, Ehsan Heidari A.3, Soleimani M.4, Jahedi Zargar M.1,
Arefian E.5, Madani T.6, Asadi M.3, Poopak B.7
 
 
1Allied-Health Faculty, Alborz University of Medical Sciences, Karaj, Iran
2Stem Cell Technology Research Center, Tehran, Iran
3Faculty of Medical Sciences, Alborz University of Medical Sciences, Karaj, Iran
4Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
5Biology School of Tehran University, Tehran, Iran
6Peyvand Clinical & Specialty Laboratory, Tehran, Iran
7Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran
 
 
 
Abstract
Background and Objectives
Acute lymphoblastic leukemia (ALL) is the most common malignancy in childhood. The prevalence of ALL is 1 to 4/7 per 100,000 population every year world-wide. Long non-coding RNAs (LncRNAs) are a group of non-coding RNAs involved in many biological processes, such as epigenetic changes, regulation of mRNA transcription and translation. Identification of their function can be helpful to understand the pathophysiology and treatment of ALL. One of the important LncRNAs in hematopoiesis regulation is HOTAIR (LncRNA HOX transcript antisense RNA). The purpose of this study was to determine the expression of HOTAIR LncRNA in newly diagnosed B-ALL patients.
 
Materials and Methods
In this cross-sectional descriptive study, 40 newly diagnosed B-ALL patients as well as 15 healthy individuals as control were given 1mL of bone marrow specimen with EDTA anticoagulant. RNA (patients and control) was extracted and Real-time quantitative PCR was used to examine the expression of LncRNA HOTAIR in ALL patients. The results was analyzed by REST 2009.
 
Results
The findings showed that expression of HOTAIR in patients with Acute Lymphoblastic Leukemia type B increased significantly as compared to healthy controls (p ˂ 0.05).
 
Conclusions 
In conclusion, the increase expression of HOTAIR in acute lymphoblastic leukemia can be attributed to potential role of LncRNA HOTAIR in pathophysiology of the disease.
 
Key words: Hematopoiesis, LncRNAs, Lymphocytic Leukemia، Acute
 
Received:  22 Aug 2017
Accepted: 13 Feb 2018
 
 

Correspondence: Poopak B., PhD of Hematology and Blood Banking. Assistant Professor of Islamic Azad University, Tehran Medical Sciences Branch.
P.O.Box: 19395-1495, Tehran, Iran. Tel: (+9821) 22264144; Fax: (+9821) 22264144
E-mail:
1- Acridine Orange
1- Biological safety cabinet
1- Platelet Concentrate
2- Food and Drug Administration
3- Normal Skin Flora
4- Platelet Rich Plasma-Platelet Concentrate
5- Eosin-Methylene blue
6- Thioglycolate
bpoopak@gmail.com
    References:
  1. Tomizawa D, Kiyokawa N. Acute Lymphoblastic Leukemia: Hematological Disorders in Children. USA: Springer; 2017. p. 33-60.
  2. Inaba H, Greaves M, Mullighan CG. Acute lymphoblastic leukaemia. Lancet 2013; 381(9881): 1943-55.
  3. Kahn JM, Keegan TH, Tao L, Abrahão R, Bleyer A, Viny AD. Racial disparities in the survival of American children, adolescents, and young adults with acute lymphoblastic leukemia, acute myelogenous leukemia, and Hodgkin lymphoma. Cancer 2016; 122(17): 2723-30.
  4. Fatica A, Bozzoni I. Long non-coding RNAs: new players in cell differentiation and development. Nat Rev Genet 2014; 15(1): 7-21.
  5. Esteller M. Non-coding RNAs in human disease. Nat Rev Genet 2011; 12(12): 861.
  6. Boon RA, Jaé N, Holdt L, Dimmeler S. Long Noncoding RNAs: From Clinical Genetics to Therapeutic Targets? J Am Coll Cardiol 2016; 67(10): 1214-26.
  7. Sigova AA, Mullen AC, Molinie B, Gupta S, Orlando DA, Guenther MG, et al. Divergent transcription of long noncoding RNA/mRNA gene pairs in embryonic stem cells. Proceedings of the National Academy of Sciences 2013; 110(8): 2876-81.
  8. Cesana M, Cacchiarelli D, Legnini I, Santini T, Sthandier O, Chinappi M, et al. A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA. Cell 2011; 147(2): 358-69.
  9. Mercer TR, Dinger ME, Mattick JS. Long non-coding RNAs: insights into functions. Nat Rev Genet 2009; 10(3): 155.
  10. Wu Y, Zhang L, Zhang L, Wang Y, Li H, Ren X, et al. Long non-coding RNA HOTAIR promotes tumor cell invasion and metastasis by recruiting EZH2 and repressing E-cadherin in oral squamous cell carcinoma. Int J Oncol 2015; 46(6): 2586-94.
  11. Huang L, Liao LM, Liu AW, Wu JB, Cheng XL, Lin JX, et al. Overexpression of long noncoding RNA HOTAIR predicts a poor prognosis in patients with cervical cancer. Arch Gynecol Obstet 2014; 290(4): 717-23.
  12. Svoboda M, Slyskova J, Schneiderova M, Makovicky P, Bielik L, Levy M, et al. HOTAIR long non-coding RNA is a negative prognostic factor not only in primary tumors, but also in the blood of colorectal cancer patients. Carcinogenesis 2014; 35(7): 1510-5.
 
  1. Hunger SP, Mullighan CG. Acute lymphoblastic leukemia in children. N Engl J Med 2015; 373(16): 1541-52.
  2. Rinn JL, Kertesz M, Wang JK, Squazzo SL, Xu X, Brugmann SA, et al. Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs. Cell 2007; 129(7): 1311-23.
  3. Swain A, Inoue T, Tan KS, Nakanishi Y, Sugiyama D. Intrinsic and extrinsic regulation of mammalian hematopoiesis in the fetal liver. Histol Histopathol 2014; 29(9): 1077-82.
  4. Liu XH, Sun M, Nie FQ, Ge YB, Zhang EB, Yin DD, et al. LncRNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer. Mol Cancer 2014; 13(1): 92.
  5. Kim K, Jutooru I, Chadalapaka G, Johnson G, Frank J, Burghardt R, et al. HOTAIR is a negative prognostic factor and exhibits pro-oncogenic activity in pancreatic cancer. Oncogene 2013; 32(13): 1616-25.
  6. Nakagawa T, Endo H, Yokoyama M, Abe J, Tamai K, Tanaka N, et al. Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer. Biochem Biophys Res Commun 2013; 436(2): 319-24.
  7. Qiu JJ, Lin YY, Ye LC, Ding JX, Feng WW, Jin HY, et al. Overexpression of long non-coding RNA HOTAIR predicts poor patient prognosis and promotes tumor metastasis in epithelial ovarian cancer. Gynecol Oncol 2014; 134(1): 121-8.
  8. Zhang YY, Huang SH, Zhou HR, Chen CJ, Tian LH, Shen JZ. Role of HOTAIR in the diagnosis and prognosis of acute leukemia. Oncol Rep 2016; 36(6): 3113-22.
  9. Hao S, Shao Z. HOTAIR is upregulated in acute myeloid leukemia and that indicates a poor prognosis. Int J Clin Exp Pathol 2015; 8(6): 7223-8.
  10. Nagano T, Fraser P. No-nonsense functions for long noncoding RNAs. Cell 2011; 145(2): 178-81.
  11. Kogo R, Shimamura T, Mimori K, Kawahara K, Imoto S, Sudo T, et al. Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers. Cancer Res 2011; 71(20): 6320-6.
  12. Loewen G, Jayawickramarajah J, Zhuo Y, Shan B. Functions of lncRNA HOTAIR in lung cancer. J Hematol Oncol 2014; 7: 90.
  13. Wu B, Ma H, Qu W, Wang B, Zhang X, Wang P, et al. Catalytic infrared and hot air dehydration of carrot slices. J Food Process Eng 2014; 37(2): 111-21.
  14. Yan TH, Lu SW, Huang YQ, Que GB, Chen JH, Chen
YP, et al. Upregulation of the long noncoding RNA HOTAIR predicts recurrence in stage Ta/T1 bladder cancer. Tumour Biol 2014; 35(10): 10249-57.
 
 
 


 
 
 
 
Sci J Iran Blood Transfus Organ 2018; 15(1): 28-35
Original Article
 

 

HOTAIR expression in newly diagnosed Acute Lymphoblastic
 Leukemia pateints
 
Fallah P.1, Molaie M.2, Ehsan Heidari A.3, Soleimani M.4, Jahedi Zargar M.1,
Arefian E.5, Madani T.6, Asadi M.3, Poopak B.7
 
 
1Allied-Health Faculty, Alborz University of Medical Sciences, Karaj, Iran
2Stem Cell Technology Research Center, Tehran, Iran
3Faculty of Medical Sciences, Alborz University of Medical Sciences, Karaj, Iran
4Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
5Biology School of Tehran University, Tehran, Iran
6Peyvand Clinical & Specialty Laboratory, Tehran, Iran
7Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran
 
 
 
Abstract
Background and Objectives
Acute lymphoblastic leukemia (ALL) is the most common malignancy in childhood. The prevalence of ALL is 1 to 4/7 per 100,000 population every year world-wide. Long non-coding RNAs (LncRNAs) are a group of non-coding RNAs involved in many biological processes, such as epigenetic changes, regulation of mRNA transcription and translation. Identification of their function can be helpful to understand the pathophysiology and treatment of ALL. One of the important LncRNAs in hematopoiesis regulation is HOTAIR (LncRNA HOX transcript antisense RNA). The purpose of this study was to determine the expression of HOTAIR LncRNA in newly diagnosed B-ALL patients.
 
Materials and Methods
In this cross-sectional descriptive study, 40 newly diagnosed B-ALL patients as well as 15 healthy individuals as control were given 1mL of bone marrow specimen with EDTA anticoagulant. RNA (patients and control) was extracted and Real-time quantitative PCR was used to examine the expression of LncRNA HOTAIR in ALL patients. The results was analyzed by REST 2009.
 
Results
The findings showed that expression of HOTAIR in patients with Acute Lymphoblastic Leukemia type B increased significantly as compared to healthy controls (p ˂ 0.05).
 
Conclusions 
In conclusion, the increase expression of HOTAIR in acute lymphoblastic leukemia can be attributed to potential role of LncRNA HOTAIR in pathophysiology of the disease.
 
Key words: Hematopoiesis, LncRNAs, Lymphocytic Leukemia، Acute
 
Received:  22 Aug 2017
Accepted: 13 Feb 2018
 
 

Correspondence: Poopak B., PhD of Hematology and Blood Banking. Assistant Professor of Islamic Azad University, Tehran Medical Sciences Branch.
P.O.Box: 19395-1495, Tehran, Iran. Tel: (+9821) 22264144; Fax: (+9821) 22264144
E-mail:
1- Acridine Orange
1- Biological safety cabinet
1- Platelet Concentrate
2- Food and Drug Administration
3- Normal Skin Flora
4- Platelet Rich Plasma-Platelet Concentrate
5- Eosin-Methylene blue
6- Thioglycolate
bpoopak@gmail.com
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Fallah P, Molaie M, Ehsan Heidari A, Soleimani M, Jahedi Zargar M, Arefian E, et al . HOTAIR expression in newly diagnosed Acute Lymphoblastic Leukemia pateints. Sci J Iran Blood Transfus Organ 2018; 15 (1) :28-35
URL: http://bloodjournal.ir/article-1-1139-en.html


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Volume 15, Issue 1 (Spring 2018) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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