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:: Volume 14, Issue 3 (Atumn 2017) ::
Sci J Iran Blood Transfus Organ 2017, 14(3): 175-187 Back to browse issues page
The antioxidant impact of the L-carnitine (LC) in platelet concentrates during storage
N. Helmi Siasi , M.R. Deyhim , F. Amiri , S. Amini Kafiabad
Keywords: Key words: Platelets, Oxidative Stress, L-Carnitine
Full-Text [PDF 670 kb]   (1301 Downloads)     |   Abstract (HTML)  (4894 Views)
Type of Study: Research | Subject: Blood transfusion medicine
Published: 2017/09/11
Full-Text:   (1976 Views)
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Sci J Iran Blood Transfus Organ 2017; 14(3): 175-187
Original Article
 

 

The antioxidant impact of the L-carnitine (LC) in platelet
concentrates during storage
 
Helmi Siasi N.1, Deyhim M.R.1, Amiri F.1, Amini Kafi-Abad S.1
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
 
 
 
Abstract
Background and Objectives
Oxidative stress has a crucial role in formation of platelet storage lesion (PSL) which can lead to decreased platelet viability and quality for transfusion. In this study, we aimed to evaluate the L-carnitine (LC) antioxidant properties on platelet oxidative damage during storage.
 
Materials and Methods
In this experimental study, we selected ten platelet concentrated (PC) bags from healthy donors prepared from Iranian Blood Transfusion Organization (IBTO) and stored at 22º C with gentle agitation up to 5 days in the presence or absence of a 100 mM L-carnitine. For evaluation of oxidative damage, we measured total antioxidant capacity (TAC) and malondealdehyde concentration (MDA) in L-carnitine treated platelets and untreated platelets (control group); then, the results were compared between th etwo groups.
 
Results
According to the results, TAC in LC treated platelets was significantly higher than platelets without LC on day 3 and day 5 of storage (p day3 = 0.007, p day5 = 0.005). On the other hand, MDA concentration in LC treated platelets was less statistically significant compared with the control group on day 3 and 5 of storage (p day3 = 0.008, p day5 = 0.005).
 
Conclusions 
According to our results, LC is able to decrease lipid peroxidation in platelets. What is more, the antioxidant effect of LC could modulate oxidative damage in platelets during storage. It seems that LC can be considered as a good additive solution in platelets to maintain platelet quality.
 
Key words: Platelets, Oxidative Stress, L-Carnitine
 
 
 
 
 
 
 
 
Received:  24 Apr 2017
Accepted:  4 Jul  2017
 
 

Correspondence: Deyhim MR., PhD of Clinical Biochemistry. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601555; Fax: (+9821) 88601555
E-mail:mrdeyhim2017@gmail.com
 
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Helmi Siasi N, Deyhim M, Amiri F, Amini Kafiabad S. The antioxidant impact of the L-carnitine (LC) in platelet concentrates during storage. Sci J Iran Blood Transfus Organ 2017; 14 (3) :175-187
URL: http://bloodjournal.ir/article-1-1118-en.html
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Volume 14, Issue 3 (Atumn 2017) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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