[Home ] [Archive]   [ فارسی ]  
:: Main :: About us :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 14, Number 2 (Summer 2017) ::
Back to browse issues page 2017, 14(2): 126-134
Cytotoxic effect of PI3Kδ inhibitor in acute promyelocytic leukemia cells
M. Dadashi, D. Bashash *
Abstract:   (20 Views)

Abstract

Background and Objectives

PI3K/Akt pathway plays a key role in cell growth and proliferation. Constitutive activation of this pathway is detectable in 50-70% of patients with acute promyelocytic leukemia (APL). Previous studies showed that PI3K activity in APL cells is mainly due to overexpression of p110δ  isoform. In an effort to investigate the effect of PI3K inhibition in acute promyelocytic leukemia, APL-derived NB4 cells were subjected to different concentrations of Idelalisib, as a potent p110δ-specific inhibitor.

Materials and Methods

In this experimental study, we examined the effect of Idelalisib on the viability and metabolic activity of NB4 cells. Moreover, flowcytometery and quantitative RQ-PCR were applied to investigate both induction of apoptosis and transcriptional activity of apoptosis-related genes, respectively.

Results

The results revealed that Idelalisib not only decreased cell viability and metabolic activity in a dose- and time-dependent manner, but also induced cell death through the apoptotic pathway in NB4 cells. Our data also delineated that Idelalisib-induced apoptosis was mediated through induction of Bax and PUMA coupled with decreased expression of Mcl-1.

Conclusions

Based on P110δ activity in APL patients and the potent efficacy of Idelalisib in NB4 cells, it is tempting to suggest this inhibitor, as either single agent or in combination with common medications, for the treatment of patients with APL.

Keywords: Key words: Acute Promyelocytic Leukemia, Inhibition, Apoptosis
Full-Text [XPS 4105 kb]   (11 Downloads)    
Type of Study: Research | Subject: Hematology
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Dadashi M, Bashash D. Cytotoxic effect of PI3Kδ inhibitor in acute promyelocytic leukemia cells. Scientific Journal of Iran Blood Transfus Organ. 2017; 14 (2) :126-134
URL: http://bloodjournal.ir/article-1-1082-en.html
Back to browse issues page Volume 14, Number 2 (Summer 2017)
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
Persian site map - English site map - Created in 0.056 seconds with 782 queries by yektaweb 3388