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:: Volume 14, Issue 2 (Summer 2017) ::
Sci J Iran Blood Transfus Organ 2017, 14(2): 126-134 Back to browse issues page
Cytotoxic effect of PI3Kδ inhibitor in acute promyelocytic leukemia cells
M. Dadashi , D. Bashash *
Abstract:   (1115 Views)


Background and Objectives

PI3K/Akt pathway plays a key role in cell growth and proliferation. Constitutive activation of this pathway is detectable in 50-70% of patients with acute promyelocytic leukemia (APL). Previous studies showed that PI3K activity in APL cells is mainly due to overexpression of p110δ  isoform. In an effort to investigate the effect of PI3K inhibition in acute promyelocytic leukemia, APL-derived NB4 cells were subjected to different concentrations of Idelalisib, as a potent p110δ-specific inhibitor.

Materials and Methods

In this experimental study, we examined the effect of Idelalisib on the viability and metabolic activity of NB4 cells. Moreover, flowcytometery and quantitative RQ-PCR were applied to investigate both induction of apoptosis and transcriptional activity of apoptosis-related genes, respectively.


The results revealed that Idelalisib not only decreased cell viability and metabolic activity in a dose- and time-dependent manner, but also induced cell death through the apoptotic pathway in NB4 cells. Our data also delineated that Idelalisib-induced apoptosis was mediated through induction of Bax and PUMA coupled with decreased expression of Mcl-1.


Based on P110δ activity in APL patients and the potent efficacy of Idelalisib in NB4 cells, it is tempting to suggest this inhibitor, as either single agent or in combination with common medications, for the treatment of patients with APL.

Keywords: Key words: Acute Promyelocytic Leukemia, Inhibition, Apoptosis
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Type of Study: Research | Subject: Hematology
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Dadashi M, Bashash D. Cytotoxic effect of PI3Kδ inhibitor in acute promyelocytic leukemia cells. Sci J Iran Blood Transfus Organ. 2017; 14 (2) :126-134
URL: http://bloodjournal.ir/article-1-1082-en.html

Volume 14, Issue 2 (Summer 2017) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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